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[셀젠바이오테크:나노스트링] nCounter - Sample Types and starting Material

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 다음은 나노스트링사의 홈페이지에 있는 FAQs란의 내용을 정리하여 글을 올리겠습니다.

 

내용이 많은 관계로 Sample Types and Starting Material란의 내용만을 설명드리겠습니다.


1. What sample types can be used on the nCounter Analysis System?

The nCounter gene expression assay can use purified total RNA, raw cell lysates in guanidinium salts, blood lysate from PAXGene™, and amplified RNA directly added to the hybridization reaction. We have also obtained good results from total RNA isolated from FFPE samples, provided the RNA meets minimal integrity requirements (more than 50% greater than 300 bp) 


2. How much starting material do you need?

We suggest 100 ng of total RNA or a lysate of 10,000 cells. More or less material may be used to boost signal or reduce sample requirements, but quantitation of extremely rare transcripts may be affected when less material is used in our system.

 

3. Can I profile microRNAs on the nCounter Analysis System?

Currently we offer nCounter miRNA Expression Assay Kits for Human v3, Mouse v1.5, and Rat v1.5. We are in the process of phasing out Human v2 and earlier versions of Mouse and Rat. This product provides a sensitive, reproducible, and highly multiplexed method for detecting specific miRNAs within total purified RNA isolated from any source, including fresh or frozen cells and tissues, formalin-fixed paraffin embedded (FFPE) samples,and biological fluids.

 

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